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Project Description

Sharon Franklin and Dr. Mark Rasmussen: Scanning electron microscopic study of protozoa isolated from rumen fluid. This project examined the diversity of species of protozoa found in cows, sheep, and goats (image at right; bar = 200µm).

Procedures and Equipment

Protozoan specimens were fixed, dehydrated and dried with a Denton CPD-1 critical point drier. Organisms were placed onto carbon adhesive coated aluminum stubs and sputter-coated with a Denton Desk II Sputter coater and imaged using a JEOL 5800LV scanning electron microscope at 10 kV with an ADDA II image capture controller and SIS Pro software.

Results

Bar = 50 µm
Bar = 100 µm
Bar = 50 µm